Figure 1.

Validation of infection-on-a-chip device

(A) Schematic of infection-on-a-chip device showing primary human neutrophils (pink) inside of an endothelial cell lumen (yellow) migrating to a source of live bacteria (green). Collagen matrix (purple) surrounds the lumen.

(B) Stained images of endothelial cell lumens. Cell nuclei are stained using Hoechst (blue), F-actin is stained with Phalloidin (red) and VE-Cadherin (green) is stained with an anti-CD144 antibody. Scale bar is 100 μm.

(C) Bacterial CFUs at 0 and 16 h for P. aeruginosa, S. enterica, L. monocytogenes, and S. aureus. Data are represented as mean ± SEM.