Table 5.

Gene expression of key enzymes and receptors of the renin-angiotensin-aldosterone system, in kidney cortex tissue from mice receiving chronic treatment with vehicle, low-dose (400 ng/kg/min), or high-dose (1,000 ng/kg/min) angiotensin II for 4 weeks.

Treatment	n	ACE1	ACE2	AT1aR	AT1bR	AT2R	Mas1
Vehicle	5–7	1.0 ± 0.1	1.0 ± 0.2	1.0 ± 0.1	1.0 ± 0.2	1.0 ± 0.2	1.0 ± 0.1
Low-dose angiotensin II	6–7	1.0 ± 0.0	2.4 ± 0.5*	2.0 ± 0.2*	1.5 ± 0.3	0.9 ± 0.2	2.0 ± 0.5*
High-dose angiotensin II	6	0.6 ± 0.1*#	0.8 ± 0.1#	4.3 ± 0.5*#	1.3 ± 0.2	0.5 ± 0.0*	1.2 ± 0.3

ACE: angiotensin converting enzyme; AT1aR: angiotensin type 1a receptor; AT1bR: angiotensin type 1b receptor; AT2R: angiotensin type 2 receptor.

Data are presented as fold increase in gene expression compared to vehicle-treated control group (2^-∆∆Ct). All values are means ± SEM.

^*denotes P < 0.05 compared to vehicle-treated group, and

^#denotes P < 0.05 compared to low-dose treatment.