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. 2023 Dec 29;67:10.29219/fnr.v67.10292. doi: 10.29219/fnr.v67.10292

Fig. 4.

Fig. 4

The effect of total alkaloids bulbus of Fritillaria cirrhosa (BFC-TA) on levels of E-cadherin mRNA, α-SMA, COL-1 protein and HYP content in rat lung tissue. (A) The mRNA expression levels of E-cadherin in lung tissue by qPCR; (B) The mRNA expression levels of Fibronectin in lung tissue by qPCR; (C) The content of HYP in lung tissue; (D) The protein exression levels of Fibronectin, E-cadherin, Collagen I and α-SMA in lung tissue were analyzed by Western blotting; (E) Quantitative analysis of protein expression levels of Fibronectin by Western blotting. Relative ratios of the indicated protein expression were normalized to GADPH and expressed as mean values; (F) Quantitative analysis of protein expression levels of Collagen I by Western blotting; (G) Quantitative analysis of protein expression levels of E-cadherin by Western blotting; (H) Quantitative analysis of protein expression levels of E-cadherin by Western blotting. Western blotting and qPCR were repeated at least 3 times (n = 3). And only a representative Western blotting result was placed in Fig. 4D. Data are presented as mean ± standard deviation. ####P < 0.0001, ###P < 0.001, ##P < 0.01 versus the NC group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus the BLM group.