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. 2023 Dec 29;67:10.29219/fnr.v67.10292. doi: 10.29219/fnr.v67.10292

Fig. 5.

Fig. 5

The effect of total alkaloids bulbus of Fritillaria cirrhosa (BFC-TA) on pro-inflammatory factors, anti-inflammatory factors in rat serum and NF-κB signaling pathway proteins in lung tissue. (A) Serum TNF-α concentration was determined by Elisa; (B) Serum IL-1β concentration was determined by Elisa kit; (C) Serum IL-6 concentration was determined by Elisa kit; (D) Serum IL-18 concentration was determined by Elisa kit; (E) Serum IL-4 concentration was determined by Elisa kit; (F) serum IL-10 concentration determined by Elisa kit; (G) The protein expression levels of p-NF-κB, NF-κB and IκBα in lung tissue were analyzed by Western blotting; (H) Quantitative analysis of protein expression levels of p-NF-κB/NF-κB by Western blotting. Relative ratios of the indicated protein expressions were normalized to GADPH and expressed as mean values; (I) Quantitative analysis of protein expression levels of IκBα by Western blotting. Serum data are presented as mean ± standard deviation (n = 12). Western blotting were repeated at least 3 times (n = 3), and only a representative result in Fig. 5G. Data are presented as mean ± standard deviation. ####P < 0.0001, ###P < 0.001 versus the NC group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus the BLM group.