Fig. 6.

The effect of total alkaloids bulbus of Fritillaria cirrhosa (BFC-TA) on transforming growth factor-β (TGF-β)/Smad signaling pathways in rats. (A) Elisa kit was used to determine the content of TGF-β1 in rat serum; (B) The protein expression levels of p-Smad2 and Smad2 in lung tissue were analyzed by Western blotting; (C) Quantitative analysis of protein expression of p-Smad2/Smad2 by Western blotting. Relative ratios of the indicated protein expressions were normalized to GADPH and expressed as mean values. Serum data are presented as mean ± standard deviation (n = 12). Western blotting were repeated at least 3 times (n = 3), and only a representative result in Fig. 6B. Data are presented as mean ± standard deviation. ^###P < 0.001, ^##P < 0.01 versus the NC group. *P < 0.05, **P < 0.01 versus the BLM group.