REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Viobility Fixable Dye 405/520	Miltenyi Biotec	120-028-574
CD4–APC-Vio770	Miltenyi Biotec	Cat# 130-113-223; RRID: AB_2726034
CD8–APC	Miltenyi Biotec	Cat# 130-110-679; RRID: AB_2659237
HLA-DR–VioBlue	Miltenyi Biotec	Cat# 130-111-794; RRID: AB_2652162
CD38–PE-Vio770	Miltenyi Biotec	Cat# 130-113-432; RRID: AB_2733228
Granzyme-B–PE	Miltenyi Biotec	Cat# 130-116-486; RRID: AB_2727564
Perforin–FITC	Miltenyi Biotec	Cat# 130-118-189; RRID: AB_2733686
CD8–PerCP-Vio700	Miltenyi Biotec	Cat# 130-110-682; RRID: AB_2659249
IL-17A–FITC	Miltenyi Biotec	Cat# 130-120-410; RRID: AB_2752083
IL-4–PE	Miltenyi Biotec	Cat# 130-123-698; RRID: AB_2905285
TNF-α–PE-Vio770	Miltenyi Biotec	Cat# 130-120-492; RRID: AB_2784483
IFN-γ–VioBlue	Miltenyi Biotec	Cat# 130-119-577; RRID: AB_2751736
IL-2–APC	Miltenyi Biotec	Cat# 130-111-304; RRID: AB_2652419
Goat anti-human k light chain antibody biotinylated	Bethyl Laboratories	Cat# A80-115B; RRID: AB_1966048
Goat anti-human λ light chain antibody biotinylated	Bethyl Laboratories	Cat# A80-116B; RRID: AB_1966050
Human Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody, clone BIB116	Creative Diagnostics	CABT-CS044
Biological samples
PBMCs	This study	N/A
Plasma	This study	N/A
Chemicals, peptides, and recombinant proteins
PepTivator® SARS-CoV-2 Prot_S	Miltenyi Biotec	130-126-700
PepTivator® SARS-CoV-2 Prot_N	Miltenyi Biotec	130-126-698
PepTivator® SARS-CoV-2 Prot_M	Miltenyi Biotec	130-126-702
Phorbol myristate acetate (PMA)	Sigma-Aldrich	16561-29-8
Ionomycin	Sigma-Aldrich	56092-82-1
Brefeldin A	ThermoFisher	00-4506-51
Paraformaldehyde solution, 4% in PBS	ThermoFisher	15670799
Saponin	Sigma-Aldrich	8047-15-2
Carbonate-bicarbonate	Sigma-Aldrich	C3041-50cap
Avidin-HRP	ThermoFisher	18-4100-51
3,3’,5,5’-tetramethylbenzidine (TMB)	Invitrogen	229280010
H2SO4	Sigma-Aldrich	4803641000
Soluble human angiotensin-converting enzyme 2 (ACE2) protein fused to a human IgG1 Fc tag	InvivoGen	Fc-hace2
SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)	ACROBiosystems	SPD-C52H3
Recombinant wild-type SARS-CoV-2 RBD-HRP	Biaffin GmbH & Co KG	SPD-SR2H0-200
Lymphosep, Lymphocyte Separation Media	Biowest	L0560
Dimethyl Sulfoxide	EuroClone	67-68-5
Critical commercial assays
MACSPlex Cytokine 12 kit, human	Miltenyi Biotec	130-099-169
QIAamp Viral RNA Mini Kit	QIAGEN	221413
Oligonucleotides
CDC 2019-nCoV_N1 primers and probe set	Centers for Disease Control and Prevention, CDC	N/A
TaqPath™ 1-Step RT-qPCR Master Mix CG	ThermoFisher	A15299
Software and algorithms
FlowLogic 8	Inivai Technologies	N/A
FlowJo 10.8	BD Biosciences	N/A
Prism 9.4	GraphPad by Dotmatics	N/A
SPICE 6.0	National Institutes of Health	N/A
Other
Vacutainer™ EDTA	BD Biosciences	367525
High-binding 96-well plates	Greiner Bio-One	655061
Fetal bovine serum	EuroClone	ECS0180L
Bovine serum albumin	Sigma-Aldrich	A7030-100G
RPMI 1640 w/o L-Glutamine	EuroClone	ECB2000
PBS Dulbecco's w/o Calcium w/o Magnesium	EuroClone	ECB4004
Penicillin-Streptomycin	Sigma	P4333-100ML
L-Glutamine	Gibco	25030-149
TWEEN® 20	Sigma	P9416-100ML
FACSVerse™	BD Biosciences	N/A
Sunrise™	TECAN	N/A
EnSight™ Multimode Plate Reader	PerkinElmer	N/A