TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristicsa | Origin (reference) |
|---|---|---|
| S. aureus | ||
| RN4220 | Restriction negative, modification positive | R. Novick 40 |
| 8325-4 | Wild-type strain cured of known prophages | R. Novick 48 |
| PC6911 | agrΔ::tetM Tcr | Laboratory stock 12, 51 |
| PC2429 | sarA+ sarA::km Kmr Eryr | This study |
| PC1839 | sarA::km Kmr | This study |
| PC18391 | sarA::km agrΔ::tetM Kmr Tcr | This study |
| PC322 | hla+ hla::lacZ Eryr | Laboratory stock 12 |
| PC3221 | hla+ hla::lacZ sarA::km Eryr Kmr | This study |
| PC324 | hla+ hla::lacZ agrΔ::tetM Eryr Tcr | Laboratory stock 12 |
| PC3241 | hla+ hla::lacZ sarA::km agrΔ::tetM Eryr Kmr Tcr | This study |
| PC203 | spa+ spa::lacZ Eryr | Laboratory stock 12 |
| PC2031 | spa+ spa::lacZ sarA::km Eryr Kmr | This study |
| PC206 | spa+ spa::lacZ agrΔ::tetM Eryr Tcr | Laboratory stock 12 |
| PC2062 | spa+ spa::lacZ sarA::km agrΔ::tetM Eryr Kmr Tcr | This study |
| PC1072 | tst::lux Tcr | Laboratory stock 12 |
| PC1091 | tst::lux sarA::km Tcr Kmr | This study |
| S6 | Enterotoxin-producing strain | S. Khan 20 |
| PC1700 | agrΔ::tetM in S6 Tcr | This study |
| PC1841 | sarA::km in S6 Kmr | This study |
| PC1845 | sarA::km agrΔ::tetM in S6 Tcr Kmr | This study |
| E. coli DH5α | φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | Promega Corp. |
| Plasmids | ||
| pUBS1 | E. coli cloning vector; Apr | G. Murphy 25 |
| pAZ106 | Promoterless, transcriptional lacZ fusion vector; Apr (E. coli) Eryr (S. aureus) | A. Moir 38 |
| pDG783 | 1.5-kb kanamycin resistance cassette in pSB118; Apr | P. Stragier 29 |
| pPC905 | 1.3-kb SalI-BamHI cut PCR fragment containing the complete sar operon in SalI-BamHI-cut pUBS1; Apr | This study |
| pH4 | 1.5-kb EcoRI fragment from pDG783 containing the kanamycin resistance cassette in EcoRI-cut pPC905; Apr | This study |
| pH4A5 | 3.3-kb PvuII fragment containing sarA::km in SmaI-cut pAZ106; Apr (E. coli) Eryr (S. aureus) | This study |
a
Abbreviations: Tcr, Kmr, Eryr, and Apr, resistance to tetracycline, kanamycin/neomycin, erythromycin/lincomycin, and ampicillin, respectively.