Figure 2.

Auto-Abs neutralize all 12 IFN-α subtypes and the glycosylated IFNs. (A) Results for the neutralization of 12 individual IFN-α subtypes, IFN-ω or IFN-β, from children with auto-Abs (n = 8), healthy controls (n = 2), or auto-Ab positive patients (n = 2). Relative luciferase activity is shown (ISRE dual luciferase activity, with normalization against Renilla luciferase activity, and to the non-stimulated [NS] condition) after stimulation with the various type I IFNs at a concentration of 1 ng/ml in the presence of plasma (1/10 dilution). All samples were tested once. RLA: relative luciferase activity. (B–E) Plots representing the neutralization results for glycosylated (at 1 ng/ml) or unglycosylated (at 10 ng/ml or 100 pg/ml) forms of IFN-α2 and IFN-ω. The dots in the lower right part of the plot indicate neutralization of the glycosylated form of the IFN but not of the unglycosylated form, whereas the dots in the upper right part of the plot indicate neutralization of the unglycosylated form of the IFN but not of the glycosylated form. The dots in the lower left part of the plot indicate the neutralization of either form of the IFNs, whereas those in the upper right part of the plot indicate an absence of neutralization for both forms; (B) unglycosylated form of IFN-α2 at 10 ng/ml; (C) unglycosylated form of IFN-α2 at 100 pg/ml; (D) unglycosylated form of IFN-ω at 10 ng/ml; and (E) unglycosylated form of IFN-ω at 100 pg/ml.