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. 2023 Jun 20;57(1):e13522. doi: 10.1111/cpr.13522

FIGURE 3.

FIGURE 3

Overexpression of Wnt10b improved the osteogenesis ability of OP‐ASCs by activating canonical Wnt signalling pathway in vitro. (A, C) After Wnt10b transduction, the mRNA expression levels of β‐catenin, Lef1, Runx2 and OPN were greatly upregulated and downregulated by Lenti‐Wnt10b and Lenti‐shRNAWnt10b, respectively, on Days 3 and 7 of osteogenic induction (**p < 0.05 and ***p < 0.001). (B, D) The protein levels of β‐catenin, Lef1, Runx2 and OPN showed the same pattern as seen with mRNA expression (*p < 0.05, **p < 0.01 and ***p < 0.001). (E) Alizarin red staining on Day 14 shows the calcium deposition formed by the OE‐Wnt10b group was significantly more than the Mock group, but the calcium deposition that emerged in the KD‐Wnt10b group was the least among the three groups. Data represent the mean ± SD of at least three independent experiments (*p < 0.05, **p < 0.01 and ***p < 0.001). OP‐ASC, osteoporotic adipose‐derived stem cell; OPN, osteopontin.