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. 2023 Aug 8;57(1):e13535. doi: 10.1111/cpr.13535

FIGURE 6.

FIGURE 6

TDH inhibits LPS‐induced ROS levels in vitro. (A) DCF was used to measure intracellular ROS levels. (B, C) DCF fluorescence intensities on cells per well were quantified. (D) The protein expressions of Nrf2, Keap1 and Hmox1 were assessed by Western blot. (E–H) The ratios of Nrf2/Keap1 and Nrf2, Keap1 and Hmox1 to β‐actin (n = 3). All statistical histograms were expressed as means and standard deviations. *p < 0.05, **p < 0.01, ***p < 0.001. DCF, 2′,7′‐dichlorofluorescein; HO‐1, heme oxygenase 1; Keap1, Kelch‐like ECH‐associated protein; Nrf2, nuclear factor erythroid‐derived 2‐like 2; RANKL, receptor activator of the nuclear factor‐κB ligand; ROS, reactive oxygen species; TDH, Tenacissoside H.