TABLE 2.
Growth-phase-dependent expression of highly-RpoS-dependent promoter-lacZ fusions in strains grown in rich mediuma
| Mutation | Identified gene | β-Galactosidase activity (Miller units)
|
|||||
|---|---|---|---|---|---|---|---|
| Exponential phase
|
Stationary phase
|
||||||
| Wild type | rpoS13::Tn10 | RpoS dependence | Wild type | rpoS13::Tn10 | RpoS dependence | ||
| rsd1010 | gabP | 6.3 ± 0.7 | 0.4 ± 0.1 | 15 | 116.7 ± 8.8 | 1.3 ± 0.0 | 92.0 |
| rsd1014 | katE | 1.6 ± 0.0 | 0.4 ± 0.0 | 3.6 | 60.0 ± 2.0 | 1.2 ± 0.0 | 52.0 |
| rsd1035 | o381 | 6.1 ± 0.6 | 0.7 ± 0.0 | 8.2 | 52.6 ± 7.7 | 1.6 ± 0.1 | 33.7 |
| rsd1047 | f786 | 2.3 ± 0.1 | 0.3 ± 0.0 | 8.6 | 40.1 ± 1.6 | 0.8 ± 0.0 | 52.4 |
| rsd1076 | o371 | 11.0 ± 0.8 | 1.5 ± 0.3 | 7.5 | 137.4 ± 3.9 | 2.7 ± 0.1 | 50.9 |
| rsd1081 | yhiU | 6.3 ± 0.5 | 1.0 ± 0.2 | 6.2 | 108.0 ± 0.3 | 2.1 ± 0.4 | 50.7 |
| rsd1091 | osmY | 8.6 ± 0.9 | 3.0 ± 0.2 | 2.9 | 408.8 ± 27.8 | 6.0 ± 0.4 | 67.6 |
| rsd1093 | o215 | 4.5 ± 0.1 | 0.4 ± 0.2 | 10.4 | 60.4 ± 1.3 | 1.3 ± 0.3 | 45.0 |
| rsd1095 | ecnB | 11.3 ± 0.9 | 3.1 ± 1.0 | 3.8 | 152.8 ± 12.2 | 7.5 ± 0.1 | 20.4 |
| rsd1098 | otsA | 10.7 ± 0.4 | 1.2 ± 0.3 | 9.1 | 158.3 ± 8.3 | 1.4 ± 0.1 | 115.3 |
a
Strains were grown overnight in LB broth, subcultured, and maintained in the exponential phase for 8 generations prior to sampling. Cultures were sampled at the exponential phase (OD600 = 0.3) and stationary phase (OD600 = 1.6).