TABLE 3.

Growth-phase-dependent expression of highly-RpoS-dependent promoter-lacZ fusions in strains grown in glucose minimal medium^a

Mutation	Identified gene	β-Galactosidase activity (Miller units)
		Exponential phase			Stationary phase
		Wild type	rpoS13::Tn10	RpoS dependence	Wild type	rpoS13::Tn10	RpoS dependence
rsd1010	gabP	8.5 ± 0.2	0.9 ± 0.1	9.0	43.0 ± 9.0	1.4 ± 0.0	30.5
rsd1014	katE	7.9 ± 1.4	0.5 ± 0.1	15.4	53.8 ± 6.8	1.3 ± 0.1	42.7
rsd1035	o381	14.4 ± 0.9	0.6 ± 0.0	23.2	66.4 ± 3.4	0.9 ± 0.1	74.2
rsd1047	f786	5.1 ± 1.1	0.5 ± 0.1	10.6	38.3 ± 3.2	0.6 ± 0.1	63.0
rsd1076	o371	9.8 ± 0.7	1.0 ± 0.1	9.6	72.2 ± 7.4	1.9 ± 0.1	40.1
rsd1081	yhiU	13.2 ± 1.2	1.6 ± 0.2	8.1	53.1 ± 0.3	3.0 ± 0.4	17.4
rsd1091	osmY	12.8 ± 0.1	2.1 ± 0.2	6.2	107.9 ± 7.8	2.3 ± 0.1	46.3
rsd1093	o215	13.6 ± 1.0	0.7 ± 0.1	19.2	79.7 ± 15.5	0.9 ± 0.1	86.4
rsd1095	ecnB	5.9 ± 0.3	2.0 ± 0.1	2.9	95.6 ± 7.5	4.4 ± 0.2	21.8
rsd1098	otsA	10.1 ± 1.6	2.0 ± 0.1	4.8	98.2 ± 3.6	2.9 ± 0.2	34.2

^aStrains were grown overnight in minimal medium, subcultured, and maintained for 8 generations in the exponential phase prior to sampling. Cultures were sampled in the exponential phase (OD₆₀₀ = 0.2) and stationary phase (OD₆₀₀ = 0.9).