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. 2024 Jan 6;15:330. doi: 10.1038/s41467-023-44683-0

Fig. 5. CTSZ effect on hPAEC function by siRNA manipulation.

Fig. 5

a Apoptosis activation measured by caspase-3/7 activity in hPAEC following siRNA plus vehicle or indicated pro-inflammatory treatments. b Cell viability measured by CellTiterGlo assay in hPAEC 48 h following siRNA treatment and 24 h in 2%FBS-ECM plus vehicle or indicated treatments to match caspase assay. c Apoptosis and d necrosis measured by Annexin V cell surface binding and DNA leak. *P < 0.05, **P < 0.01, ***P < 0.001 versus both control siRNAs. e Proliferation estimated by metabolically active cells in MTT assay following siRNA treatment. n = 9 independent cultures from 3 independent experiments for a–e. f CTSZ expression (compared to loading controls vinculin and beta-actin) following siRNA knockdown in hPAEC by immunoblot and qPCR. siNeg negative control scrambled siRNAs, siGAPDH control siRNA targeting GAPDH. n = 3 cell cultures from 3 independent experiments. Bars indicate mean and SEM. One way ANOVA with Dunnett’s posthoc tests corrected for multiple comparisons in all figures. ***p < 0.0001 vs. other siRNAs within treatment group.