Fig. 1.

Experimental design of both dietary perturbation studies. Study 1 (yellow), exposed three groups consisting of three cages with two mice each to ad libitum chow, ad libitum high fat diet, or fasting for 16 after which animals were euthanized and samples dissected for bulk RNA-seq. Study 2 (blue), exposed two groups of 10 cages with single-housed mice each to ad libitum chow or fasting for 24 h before collecting samples for bulk RNA-seq. Abbreviations: Ad lib., ad libitum; h, hour; Inf., inferior; sup., superior.