SDS-PAGE of inner and outer membrane proteins of anaerobically grown MR-1, SR-21, and SR-21C. Lanes: 1 and 2, cell membrane fractions from MR-1 and SR-21, respectively; 3, 4, and 5, outer membrane fractions of MR-1, SR-21, and SR-21C, respectively. The band that corresponds to MtrB, which is not detected in the outer membrane fraction of the mutant SR-21, is indicated by an arrow. Numbers correspond to protein molecular mass markers (in kilodaltons).