TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5αMCR | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA supE44 λ− thi-1 gyrA96 relA1 | BRL |
| S17-1 | Pro (r− m+) contains integrated plasmid RP4-2-Tc::Mu-Km::Tn7, Tpr Smr | 42 |
| S. putrefaciens | ||
| MR-1 | Lake Oneida isolate | 28 |
| MR-1R | Spontaneous mutant of MR-1, Rifr | This work |
| SR-21 | MR-1R but freC::Tn5, Rifr Kmr | This work |
| SRC-21 | SR-21 carrying pSC52, Rifr Kmr Tcr | This work |
| Plasmids | ||
| pUC119 | Cloning and sequencing vector, Ampr | BRL |
| pSPORT1 | Cloning and sequencing vector, Ampr | BRL |
| pRK415 | IncP plamid, Tcr | 15 |
| pSUP1011 | pACYC184 derivative carrying RP4 oriT and Tn5, Cmr Kmr | 42 |
| pSRF212 | pSPORT1 with freB-containing 6.2-kb EcoRI-BamHI fragment | This work |
| pAB52 | pUC119 with freBC-containig 5.8-kb EcoRI-HindIII fragment | This work |
| pSC52 | pRK415 with freBC-containing 5.8-kb EcoRI-HindIII fragment | This work |