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. 2024 Jan 6;10:10. doi: 10.1038/s41531-023-00618-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative IHC images of coronal brain sections of cortex and substantia nigra of wild-type mice that received a single injection of an AAV9 encoding sp2- or wt-αSyn in the cortex. Animals were sacrificed at 4 months post-surgery. In the upper part a schematic representation of the anatomical structures analyzed that includes approximate rostro-caudal coordinates with the bregma as reference. Quantification is shown in (b). Data is shown as total counts per area unit ± SD. Contralateral (Contra) and ipsilateral (Ipsi) hemispheres are shown. Scale bar: 200 μm. *p < 0.05 compared to wt-αSyn. **p < 0.05 compared to ipsi (one-way ANOVA followed by the post hoc Tukey’s test).