TABLE 4.
Carbon source | Nitrogen source | GlutDH activity of:
|
|
---|---|---|---|
RocG | GudB1 | ||
Glucose | Ammonia | ≤45 | 159 |
Glucose | Glutamate | ≤45 | 178 |
Succinate | Glutamate | ≤45 | NDb |
Glucose | Glutamine | ≤45 | ND |
Succinate | Glutamine | ≤45 | ND |
Glucose | Proline | 76 | 182 |
Succinate | Proline | 67 | 391 |
Proline | Proline | 246 | 465 |
Glucose | Ornithine | 74 | 158 |
Succinate | Ornithine | 792 | 430 |
Ornithine | Ornithine | 769 | 633 |
Glucose | Arginine | 97 | 106 |
Succinate | Arginine | 998 | ND |
Arginine | Arginine | 1,040 | 662 |
Cells were grown in TSS medium. GlutDH activity was assayed and expressed in units as described in Materials and Methods. Strains BB1268 (ΔgudB::tet) and BB1284 (ΔrocG::ble gudB1) were used for determination of RocG and GudB1 activities, respectively. All numbers are averages of at least two experiments, and the mean errors did not exceed 30%.
ND, not done.