TABLE 6.
Expression of a gudB-lacZ fusion in regulatory mutants in minimal mediuma
| Genotype | β-Galactosidase activity in the presence of indicated nitrogen source
|
|
|---|---|---|
| Ammonia | Proline | |
| Wild type | 50 | 183 |
| tnrA | 58 | 179 |
| glnR | 47 | 179 |
| gltC | NGb | 209 |
| codY | 39 | 124 |
| ahrC | 90 | 160 |
| abrB | 15 | 150 |
| ccpA | 55 | 119 |
| spo0A | 62 | 150 |
| sigL | 51 | 203 |
| sigB | 35 | 151 |
| sigD | 40 | 140 |
| ΔgudB::tet | 57 | 197 |
| ΔrocG::ble ΔgudB::tet | 57 | 221 |
| gudB1 | 21 | 75 |
a
Strain BB1401 (gudB′-lacZ) and its derivatives were grown in glucose minimal medium. β-Galactosidase activity was assayed and expressed in Miller units as described in Materials and Methods. All numbers are averages of at least two experiments, and the mean errors did not exceed 20%.
b
NG, no growth under these conditions.