TABLE 1.
Bacterial strains and bacteriophages
| Strain or phage | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| N99 | galK2 galT22 rpsL IN(rrnD-rrnE)1 | VKPMa |
| C600 | supE44 hsdR thi-1 thr-1 leuB6 lacY1 tonA21 | VKPM |
| LE392 | supE44 supF58 hsdR514 galK2 galT22 metB1 trpR55 lacY | VKPM |
| XL1-blue | supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac F′[traD36 proAB+ lacIqlacZΔM15 Tn10] | VKPM |
| MW1184 | ara Δ(lac-proAB) rpsL thi (φ80 lacZΔM15) Δ(srlR-recA) 306::Tn10 | VKPM |
| K802 | supE44 hsdR2 galK2 galT22 metB1 lacY1 mcrA mcrB | VKPM |
| C600 Hfr | C600 + F′101(thr+ ara+ leu+) | VKPM |
| B3958 | K802 recA9::Tn5 | VKPM |
| B7218 | LE392 Apr | VKPM |
| B7237 | C600 hisG::Tn10 | VKPM |
| B7349 | B6905mal(λ) λ-resistant | VKPM |
| MT1 | ilv his rpsL λN7N53 cI+cro+::gal+ | 35 |
| MT2 | ilv his recA1 rpsL λN7N53 cI+cro+::gal+ | 35 |
| SG20322b | cps-11::lac-Mud1 lon-146::ΔTn10c | 3 |
| SG20645d | rcsA3 (RcsA*) zed-650::Tn10 | 3 |
| SG20797d | Δlon-510 rcsB11::ΔTn10c | 3 |
| SG20803d | rcsC137 ompC::Tn5 | 3 |
| SG20904d | Δlon-510 rcsA72::ΔTn10c | 3 |
| B6905 | C600 Δ(srlR-recA)306::Tn10 | C600 + P1(MW1184) |
| B7243 | C600 rcsA3 (RcsA*) Δ(srlR-recA)306::Tn10 | B7237 + P1(SG20645) + P1(MW1184)e |
| B7244 | N99 Δ(srl-recA)306::Tn10 | N99 + P1(MW1184) |
| B7246 | N99 dsrA::ΔTn903f | N99 + pDR301g |
| B7252 | C600 rcsA72::ΔTn10 recA9::Tn5c | C600 + P1(SG20904) + P1(B3958) |
| B7253 | C600 rcsB11::ΔTn10 recA9::Tn5c | C600 + P1(SG20797) + P1(B3958) |
| B7254 | C600 lon-146::ΔTn10 recA9::Tn5c | C600 + P1(SG20322) + P1(B3958) |
| B7307 | C600 dsrA::ΔTn903 Δ(srlR-recA)306::Tn10f | C600 + P1(B7246) + P1(MW1184) |
| B7315 | C600 rcsC137 ompC::Tn5 Δ(srlR-recA)306::Tn10 | C600 + P1(SG20803) + P1(MW1184) |
| Bacteriophages | ||
| λ+ | VKPM | |
| 21 | VKPM | |
| φ80 | VKPM | |
| 434 | VKPM | |
| Mucts62 | VKPM | |
| P1vir | VKPM | |
| λbio10 | VKPM | |
| λplac5 | VKPM | |
| λimm434bio10 | VKPM | |
| λimm434plac5 | VKPM | |
| λGE112 | b2 cI(Ind−) | 8 |
| λDY125 | b2 cI(Ind−) | 8 |
| λEK117 | b2 cI(Ind−) | 8 |
| λGR185 | b2 cI(Ind−) | 8 |
| λGE112 plac5 | cI(Ind−) | λGE112 + λimm434 plac5 |
| λDY125 plac5 | cI(Ind−) | λDY125 + λimm434 plac5 |
| λEK117 plac5 | cI(Ind−) | λEK117 + λimm434 plac5 |
| λGR185 plac5 | cI(Ind−) | λGR185 + λimm434 plac5 |
| λprecA | λcI857 recA+ | 22 |
| λ3B3 | rcsA+ dsrA+ dsrB+ | 17 |
VKPM, Russian National Collection of Industrial Microorganisms, GNIIGenetica, Moscow, Russia.
Derived from MC4100, genotype F− Δ(argF-lac)U169 araD139 rpsL150 deoC1 relA1 ptsF25 flbB5301 rbsR.
ΔTn10 (Tn10Δ16Δ17) is defective Tn10 capable of transposition but deficient in transposase (40).
Derived from MC4100 and contains cpsB10::lacMu-immλ.
B7237 + P1(SG20645) + P1(MW1184) indicates that the strain was constructed by two successive P1 vir transductions in the order indicated.
ΔTn903 contains a drug resistance marker from transposon Tn903 which encodes for an aminoglycoside 3′-phosphotransferase gene conferring kanamycin resistance (37).
N99 + pDR301 indicates that the dsrA::ΔTn903 insertion of pDR301 was transferred to the chromosome by Hfr conjugation (see Materials and Methods).