Skip to main content
. 2005 Apr 14;115(5):1163–1176. doi: 10.1172/JCI23424

Figure 5.

Figure 5

Breast tumor cell protein expression and activation differences between collagen VI+/+ and collagen VI–/– mice in the background of the MMTV-PyMT transgene. (A) Laser-capture microdissection was used to isolate hyperplastic cells in a 100-μM radius from adipocytes. A representative example is shown in the top panels, before (panel 1) and after (panel 2) removal of cells. Similarly, cells not associated at all with adipocytes (> 500 μM from the nearest adipocyte) were isolated from pathologically matched sections from mice carrying the MMTV-PyMT transgene in either a collagen VI+/+ or a collagen VI–/– background (panels 3 and 4). Arrows indicate the targeted areas before and after isolation of cells. (B) Material from at least 8 independent areas was pooled in each case and analyzed. The material was used to make protein extracts that were spotted on protein arrays. The results shown were obtained from arrays of cells in close proximity to adipocytes. Arrays were probed with various antibodies against total and activated forms of proteins implicated in pro-oncogenic pathways. *P < 0.05. (C) Quantitative comparison of the ratio of phospho-specific protein to total protein from arrays used in B. Results are shown as mean ± SEM. *P < 0.05.