FIG. 3.
(A) Nucleotide sequence of the ccpALm gene. The deduced amino acid sequence is given in single-letter code below the DNA sequence. The putative start codon is shown in bold type, and the stop codon is indicated by an asterisk. A potential ribosome-binding site (RBS) is underlined. Putative −10 and −35 sequences are double underlined. A CRE-like sequence partially overlapping the putative −35 sequence is indicated by dashed inverted arrows. A possible factor-independent transcription terminator is marked with solid inverted arrows. The site of truncation of the ccpA coding sequence in JB15 is indicated with an arrowhead. (B) Comparison of the CRE-like sequence in the ccpALm promoter region with the CRE consensus sequence (59). Ambiguity codes are as follows: W denotes A or T and N denotes A, C, G, or T. Vertical bars indicate matching bases.