FIG. 6.

CR of α-glucosidase in strains 10403S and JB15 (10403S ccpA::pCON1) in liquid medium. Cells were grown at 37°C in LB medium buffered with 100 mM MOPS (pH 7.0), supplemented with the indicated sugars (each, 25 mM). Specific activity of α-glucosidase was measured in exponentially growing cells by using p-nitrophenyl-α-d-glucoside as the substrate. Mal, maltose; Glc, glucose; Fru, fructose; Cel, cellobiose; wt, wild type. Each sample was analyzed in triplicate, and the data represent the means and standard errors of the means of three independent experiments.