TABLE 1.
Plasmids used in this study
| Plasmid | Characteristics and (or) derivationa | Source or reference |
|---|---|---|
| pAHS1 | Apr; clone, in the SalI site of pRL171 (15), of the 15-kb insert (derived from a partial Sau3AI fragment of Anabaena sp. DNA) of a λEMBL3 phage whose insert hybridizes with pRL871 | This study |
| pAHS2 | Apr; 1.7-kb fragment of Anabaena sp. genomic DNA, excised from pAHS1 with SalI (from the vector) and DraI, subcloned between the SalI and EcoRV sites of 2.67-kb plasmid pBluescript SK(+) (Stratagene) | This study |
| pAHS3 and pAHS4 | Apr; 4.2- and 3.9-kb plasmids, respectively, generated by complete digestion of pAHS2 with SalI and partial digestion with StyI, blunting with the Klenow fragment, and religation | This study |
| pAHS5, pAHS6, and pAHS7 | Smr Spr; Cmr cassette C.C1, isolated from pRL178 (15) by digestion with SalI, was inserted into the unique XhoI sites of pAHS2, pAHS3, and pAHS4, respectively, followed by transfer of a BamHI fragment containing the Anabaena sp. DNA into the BamHI site of pRL1049 (6) | This study |
| pRL386a (see Fig. 1) | Cmr Emr; to exchange Tn5-1063 for a lux cassette with a different marker, permitting second-site mutagenesis with transposon Tn5-1058 | This study |
| pRL487 | Cmr Kmr; S.K4/L.XSX1/C.C1 (nomenclature from reference 15) | 17 |
| pRL528 | Cmr; encodes methylases that modify sites targeted by the restriction endonucleases AvaI and AvaII | 14, 18 |
| pRL864 | Bmr Kmr Smr; EcoRI transposon recovery of mutant AB5 | This study |
| pRL871 | Bmr Kmr Smr; DraI transposon recovery of second-site mutant SA6 | This study |
| pRL1058 | Bmr Kmr Smr; bears transposon Tn5-1058 | 45 |
| pRL1063a | Bmr Kmr Smr; bears transposon Tn5-1063, a luxAB-containing derivative of Tn5-1058 | 45 |
| pRL1130a | Smr Spr; bears a cassette that contains sacB and aadA, whose product confers Smr and Spr | 9 |
| pRL1380 | Bmr Kmr Smr Spr; pRL871, linearized with DraI, ligated to the FspI fragment of pRL1130a that contains genes aadA and sacB | This study |
| pRL2270 | Bmr Kmr Smr; ClaI transposon recovery of mutant AB5 | This study |
| RP4 | Apr Kmr Tcr; conjugative plasmid | 42 |
a
Ap, ampicillin; Bm, bleomycin; Cm, chloramphenicol; Em, erythromycin; Km, kanamycin; Sm, streptomycin; Sp, spectinomycin; Tc, tetracycline.