FIG. 2.

Flow cytometric analysis of S. pombe cultivated under different growth conditions. (A) Media I (MM containing 0.026 g of NH₄Cl/liter), II (MM containing 5 g of NH₄Cl/liter), and III (MM containing 5 g of NH₄Cl/liter plus 100 mg of methionine/liter) were inoculated with a preculture of strain 972 (h⁻) to an optical density at 530 nm of 0.1 and incubated at 30°C. After 47 h, when cells had reached stationary phase, the cells were analyzed. Peaks of 1C and 2C DNA contents are shown. (B) Strain 972 (h⁻) was cultivated in medium I (MM containing 2 mM cAMP) and medium II (MM containing 2 mM cAMP and 200 mg of methionine/liter). Inoculation, incubation, and preparation of cells were done as described for panel A.