FIG. 2.

Identification of genomic fragments of P. putida KT2440 smaller than 15 kb after digestion with SwaI, PmeI, and SwaI plus PmeI and ³²P end labeling. Total DNA of P. putida KT2440 genomic DNA was digested with SwaI (lane S), PmeI (lane P), and SwaI plus PmeI (lane S/P) and then end labeled with ³²P as described in Materials and Methods. Fragments were separated in a conventional 0.8% (wt/vol) agarose gel run in 1× TAE buffer for 3 h at 5 V cm⁻¹. The gel was exposed to Kodak photographic film and developed. Size fragments are indicated in kilobases. Bands of interest and their sizes are indicated by arrows.