FIG. 3.

Locations of SwaI restriction sites within PmeI fragments. (A) Separation of P. putida KT2440 PmeI fragments in the first electrophoresis; (B) separation of SwaI fragments generated upon digestion of different PmeI fragments. In gel A, two different lanes of total DNA digested with PmeI were run side by side. After ethidium bromide staining of one of them (A), the agarose plugs containing the PmeI fragment of interest were excised and digested with SwaI. Finally DNA fragments were separated in the second electrophoresis (B). Lanes: 1, phage lambda DNA undigested and digested with HindIII; 9, phage lambda DNA concatemers: S, P. putida KT2440 genomic DNA digested with SwaI. Lanes 2, 3, 4, 5, 6, 7, and 8 correspond to fragments PmeI-A, PmeI-B, PmeI-C, PmeI-D, PmeI-E, PmeI-F, and PmeI-G, respectively, digested with SwaI. Sizes are indicated in kilobases.