FIG. 4.

Hybridization analysis with several gene probes. P. putida KT2440 DNA was digested with SwaI (lanes S), PmeI (lanes P), and SwaI plus PmeI (lanes 1), and DNA fragments were separated by CHEF electrophoresis. The three lanes on the left were separated from the two other lanes, and each set of DNA was transferred to a nylon membrane. In panel B, the three lanes on the left correspond to hybridization with the npt gene probe, while the other two lanes correspond to hybridization with the dnaJ-dapB-carAB set of genes. Sizes are indicated in kilobases.