TABLE 1.
Fragment sizes of all single restriction enzyme digests of SwaI, PmeI, I-CeuI, and PacI of the P. putida KT2440 chromosome
Fragment | Fragment sizea (kb) obtained with:
|
|||
---|---|---|---|---|
SwaI | PmeI | I-CeuI | PacI | |
A | 920 | 2,506 | 2,850 | 1,875 |
B | 702 | 1,025 | 1,230 | 1,750 |
C | 620 | 778 | 946 | 1,428 |
D | 584 | 729 | 610 | 678 |
E | 533 | 674 | 330 | 155 |
F | 470 | 170 | 126 | 132 |
G | 395b | 78 | ||
H | 352 | 19 | ||
I | 325 | 18 | ||
J | 180 | |||
K | 115 | |||
K′ | 105 | |||
L | 65b | |||
M | 55 | |||
N | 30 | |||
N′ | 29 | |||
O | 20 | |||
Estimated total genome size (kb) | 5,960 | 5,997 | 6,092 | 6,018 |
Average of at least 10 independent gels. Standard deviations were in the range of 1 to 5% of the given value.
The band is a doublet.