TABLE 2.

Fragment sizes resulting from double digestion of P. putida KT2440 DNA^a

Fragment	Fragment sizeb (kb) obtained with SwaI + PmeI		Name	Fragment size (kb) obtained with PmeI + PacI
SwaI-A	920		PP1	1,390
SP1	680		PP2	1,115
SP2	560		PmeI-E	678
SwaI-E	533		PP3	548
SP3	450		PP4	510
SwaI-G	395		PP5	446
SP4	390		PP6	384
SwaI-H	352		PP7	337
SwaI-I	325		PmeI-F	180
SP5	320		PacI-E	155
SP6	172		PacI-F	132
SP7	165		PacI-G/PP8	78c
SP8	130		PmeI-H	19
SwaI-K′	105		PmeI-I	19
SwaI-L*	65c
SP9	60
SwaI-M	55
SP10	45
SP11/SwN	30*
SwaI-N′	29
SP12	21
SwaI-O	20
PmeI-H	18
PmeI-I	18
SP13	8
SP14	1
Estimated total genome size (kb)	5,977			6,068

^aThe fragments were designated SwaI, PmeI, or PacI to indicate that the corresponding SwaI, PmeI, or PacI fragment was not cut by SwaI, PmeI, or PacI, respectively. The fragments resulting from the cut of a SwaI or PmeI fragment by the other enzyme are designated SP followed by a number (1 to 14) such that the higher the number, the smaller the DNA fragment. Accordingly, fragments were designated PP followed by a number when they resulted from the digestion of a PmeI fragment by PacI and vice versa. 

^bAverage of at least three independent gels. Standard deviations are in the range of 1 to 5% of the given values. 

^cThe band is a doublet.