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Localization of the Kmr determinant in the chromosomes of Kmr derivatives of P. putida EEZ15K-xa
| Kmr mutant | Fragment
|
||
|---|---|---|---|
| SwaI | PmeI | I-CeuI | |
| EEZ15K-3 | I | A | F |
| EEZ15K-8 | K′ | B | A |
| EEZ15K-12 | D | A | D |
| EEZ15K-14 | I | A | E |
| EEZ15K-16 | H | A | C |
| EEZ15K-17 | C | D | A |
| EEZ15K-22 | B | C | B |
| EEZ15K-23 | L | A | E |
| EEZ15K-24 | G | E | A |
| EEZ15K-30 | A | A | C |
Conditions for digestion and separation of fragments were as described in the legend for Fig. 1A, B, and D.
