REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD163 (Human MIF)	Leica Biosystems	CD163-L-CE; RRID: AB_2920861
CD206 (Human MIF)	Abcam	Ab64693; RRID: AB_1523910
Pan-Cytokeratin (Human MIF)	Agilent	M3515; RRID: AB_2132885
E-cadherin (Human MIF)	Agilent	M3612; RRID: AB_2076672
E-cadherin (Mouse MIF)	Thermo Fisher Scientific	13–1900; RRID: AB_2533005
Vimentin (Mouse MIF)	Thermo Fisher Scientific	PA5-27231; RRID: AB_2544707
Pan-Cytokeratin (Mouse MIF)	Abcam	ab6401; RRID: AB_305450
Pan-Cytokeratin (Mouse MIF)	Dako	M3515; RRID: AB_2132885
AF488-CD206 (Mouse MIF)	Biolegend	141709; RRID: AB_10933252
COX-2 (Mouse MIF, IHC)	Abcam	ab15191; RRID: AB_2085144
MACH 2 Mouse HRP-Polymer (IHC)	Biocare Medical	MHRP520
MACH 2 Rabbit HRP-Polymer (IHC)	Biocare Medical	RHRP520
Rat-on-Mouse HRP-Polymer (IHC)	Biocare Medical	RT517
MACH 2 Rabbit AP-Polymer (IHC)	Biocare Medical	RALP525
mCherry (IHC-P)	Thermo Fisher Scientific	PA5-34974; RRID: AB_2552323
Pimonidazole (IHC-F)	Hypoxyprobe	HP1-100; RRID: AB_2811309
COX-2 (Human IHC)	Abcam	Ab15191; RRID: AB_2085144
Cytokeratin14 (IHC)	Biolegend	905303; RRID: AB_2734678
Cytokeratin17 (IHC)	Novus	NBP1-32824; RRID: AB_2133042
F4/80 (IHC)	Genetex	GTX26640; RRID: AB_385952
CD206 (IHC)	Abcam	ab64693; RRID: AB_1523910
VEGF (IHC)	Thermo Fisher Scientific	PA5-85171; RRID: AB_2792317
CD31 (MIF)	R&D Systems	AF3628; RRID: AB_2161028
TGF-β1 (IF)	MilliporeSigma	SAB4502954; RRID: AB_10747473
VEGF-A (IF)	Thermo Fisher Scientific	MA5-13182; RRID: AB_10981661
E-cadherin (IF, WB)	Thermo Fisher Scientific	13–1900; RRID: AB_2533005
N-cadherin (IF, WB)	BD Transduction Laboratories	610920; RRID: AB_2077527
Arginase-1 (WB)	Cell Signaling Technology	93668; RRID: AB_2800207
GAPDH (WB)	MilliporeSigma	G9545; RRID: AB_796208
Vimentin (WB)	BD Pharmingen	550513; RRID: AB_393716
E-cadherin (WB)	Biolegend	147306; RRID: AB_2563230
COX-1 (WB)	Cayman Chemical	160110; RRID: AB_10078135
COX-2 (WB)	Cell Signaling Technology	4842; RRID: AB_2084968
mPGES2 (WB)	Cayman Chemical	160145; RRID: AB_10894462
TGF-β1 (WB)	Bioss Antibodies	bs-0086R; RRID: AB_10856457
VEGF (WB)	Abcam	ab68334; RRID: AB_1925554
PE-CD45 (FACS, Compensation control)	Biolegend	103105; RRID: AB_312970
BV421-CD45 (FACS, Compensation control)	Biolegend	103133; RRID: AB_10899570
PE/Cy7-CD45 (FACS, Compensation control)	Biolegend	103113; RRID: AB_312978
AF647-CD45 (FACS, Compensation control)	Biolegend	103123; RRID: AB_493534
BV785-CD45 (FACS)	Biolegend	103149; RRID: AB_2564590
PE-F4/80 (FACS)	Biolegend	123109; RRID: AB_893498
BV421-CD11c (FACS)	Biolegend	117343; RRID: AB_2563099
FITC-CD11b (FACS)	Biolegend	101205; RRID: AB_312788
PE/Cy7-CD11b (FACS)	Biolegend	101215; RRID: AB_312798
AF647-MHC class II (FACS)	Biolegend	107617; RRID: AB_493526
Bacterial and virus strains
lentivirus for firefly luciferase with UBC promoter + IRES2-mCherry-IRES-puromycin	GeneCopoeia	LPP-hLUC-Lv247-050
Biological samples
Clinical breast cancer cases	OUHSC Pathology Archive	N/A
Chemicals and recombinant proteins
Matrigel GFR	Corning	356231
D-Luciferin	PerkinElmer	122799
Clodronate liposomes	Liposoma	CP-020
Celecoxib	Cayman Chemical	10008672
PF04418948	Cayman Chemical	15016
ONO8711	Cayman Chemical	14070
L798106	Santa Cruz Biotechnology	sc-204047
GW627368X	Cayman Chemical	10009162
CP544326	Cayman Chemical	22945
17-phenyl trinor Prostaglandin E2	Cayman Chemical	14810
ONO4819	Cayman Chemical	13618
Lipopolysaccharides	MilliporeSigma	L3012
IFN-γ	Tonbo Biosciences	21-8311-U020
Prostaglandin E2	Cayman Chemical	14010
Collagenase A	MilliporeSigma	10103586001
DNase I	MilliporeSigma	10104159001
Liberase	MilliporeSigma	05401119001
Indomethacin	MilliporeSigma	I7378
Protease inhibitor	MilliporeSigma	04-693-159-001
Pimonidazole	Hypoxyprobe	HP-100
Butylated Hydroxytoluene	Spectrum	B1196
Betazoid DAB Chromogen	Biocare Medical	BDB2004
Vulcan Fast Red Chromogen	Biocare Medical	FR805
Cytosine β-D-arabinofuranoside hydrochloride	MilliporeSigma	C6645
Critical commercial assays
Mouse PGE2 ELISA	Cayman Chemical	514010
Mouse VEGF ELISA	RayBiotech	ELM-VEGF-1
Mouse Free Active TGF-β1 ELISA	Biolegend	437707
Hypoxisense 680	PerkinElmer	NEV11070
Deposited data
RNA-sequence of mouse macrophage	This paper	SRA: PRJNA1031635
Experimental models: Cell lines
Py230 murine breast cancer cell line	Gift from Dr. Lesley G. Ellies (University of California, San Diego, CA, USA)	N/A
Py2T murine breast cancer cell line	Gift from Dr. Gerhard Christofori (University of Basel, Basel, Switzerland)	N/A
NCTC clone 929 (L-929)	ATCC	CCL-1
bEnd.3 murine endothelial cell line	ATCC	CRL-2299
Experimental models: Organisms/strains
C57BL/6J (B6)	The Jackson Laboratory	Strain#000664
FVB/NJ (FVB)	The Jackson Laboratory	Strain#001800
B6.Cg-Tg(CAG-DsRed∗MST)1Nagy/J (DsRed)	The Jackson Laboratory	Sarin#006051
B6.129S4-Ptgs2tm2.1Hahe/J (Cox2luc/luc)	The Jackson Laboratory	Strain#030853
B6.129-Ptger2tm1Brey/J (EP2 KO)	The Jackson Laboratory	Strain#004376
Oligonucleotides
Primer for RT-qPCR	Integrated DNA Technologies	Table S4
Primer for Genotyping	Integrated DNA Technologies	Table S4
Software and algorithms
Living Image Analysis software version 4.7.3	PerkinElmer	N/A
GraphPad Prism software version 10.0.0	GraphPad Software	N/A
Aperio ImageScope software version 12.4.3.7001	Leica Biosystems	N/A
ZEN lite software version 3.3.89.0000	Carl Zeiss Microscopy	N/A
ImageJ software version 1.53i	National Institutes of Health	N/A
Wound healing size plugin for ImageJ		https://doi.org/10.1371/journal.pone.0232565
FlowJo software version 10.8.0	Becton Dickinson	N/A
SAS 9.4	SAS Institute	N/A
JMP 16.0.0.	SAS Institute	N/A
R version 4.1.2	R Development Core Team	N/A
BioRender	BioRender	N/A
Adobe Illustrator 28.0	Adobe	N/A