Figure 4.

Prolonged increase in muscle protein synthesis rates following ingestion of a large amount of protein

(A) Scheme demonstrating the various amino acid tracers, precursor pools, and muscle protein fractions.

(B) Scheme demonstrating the expected time resolution of the various amino acid tracers and precursor pools.

(C) Mixed-muscle protein synthesis (MPS) rates based on L-[1-¹³C]-leucine and the plasma precursor pool.

(D) MPS rates based on L-[1-¹³C]-leucine and the muscle free precursor pool.

(E) Myofibrillar protein synthesis (MyoPS) based on L-[²H₅]-phenylalanine and the plasma precursor pool.

(F) MyoPS based on L-[²H₅]-phenylalanine and the muscle free precursor pool.

(G) MyoPS based on L-[1-¹³C]-leucine and the plasma precursor pool (primary study outcome).

(H) MyoPS based on L-[1-¹³C]-leucine and the muscle free precursor pool.

(I) Muscle connective protein synthesis (ConnectivePS) based on L-[²H₅]-phenylalanine and the plasma precursor pool.

(J) ConnectivePS based on L-[²H₅]-phenylalanine and the muscle free precursor pool.

(K) ConnectivePS based on L-[1-¹³C]-leucine and the plasma precursor pool.

(L) ConnectivePS based on L-[1-¹³C]-leucine and the muscle free precursor pool.

Data in box and whiskers include the median (line), mean (cross), interquartile range (box), and minimum and maximum values (tails). For all measurements, n = 12 biological replicates.