REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Phospho mTOR (Ser 2448)	Cell Signaling	Cat#2971; AB_330970
mTor	Cell Signaling	Cat#2972; AB_330978
Phospho p70 S6 (Thr 389)	Cell Signaling	Cat#9205; AB_330944
Phospho p70 S6 (Thr 421/424)	Cell Signaling	Cat#9204; AB_2265913
P70 S6	Cell Signaling	Cat#9202; AB_331676
Phospho rS6 (Ser 235/236)	Cell Signaling	Cat#4856; AB_2181037
Phospho rS6 (Ser240/244)	Cell Signaling	Cat#2215; AB_331682
rS6	Cell Signaling	Cat#2217; AB_331355
Phospho ACC (Ser 79)	Cell Signaling	Cat#3661; AB_330337
ACC	Cell Signaling	Cat#3662; AB_2219400
Phospho 4E-BP1 (Thr 37/64)	Cell Signaling	Cat#9459; AB_330985
4E-BP1	Cell Signaling	Cat#9452; AB_331692
Myostatin	Santa Cruz	Sc134345; AB_2148785
Beclin	Cell Signaling	Cat#3738; AB_490837
LC3b	Cell Signaling	Cat#2775; AB_915950
Atg12	Cell Signaling	Cat#4180; AB_1903898
Chemicals, peptides and recombinant proteins
L-phenylalanine (1-13 C)	Cambridge Isotopes	CLM-762-MPT-PK
L-leucine (1-13 C)	Cambridge Isotopes	CLM-468-MPT-PK
L-phenylalanine (ring-D5)	Cambridge Isotopes	DLM-1258-MPT-PK
L-Tyrosine (ring-3,5-D2)	Cambridge Isotopes	DLM-449-MPT-PK
[U-13C6]-leucine	Cambridge Isotopes	CLM-2262
[U-13C915N]-phenylalanine	Cambridge Isotopes	CNLM-575
[U-13C915N]-tyrosine	Cambridge Isotopes	CNLM-439
AG 50W-X8 resin, mesh size: 100–200 μm, ionic form: hydrogen	Bio-Rad Laboratories	142–1441
MTBSTFA	Merc	8.14219
Metabolomics Amino Acid Standard	Cambridge Isotopes	MSK-A2-S
Amino Acid Standards Basics	Sigma-Aldrich	A6282
Amino Acid Standards Neutrals + Basics	Sigma-Aldrich	A6407
Accq-Taq ultra derivatization	Waters	186003836
FOXO1 taqman probe	Fisher Emergo	Hs01054576_m1
MuRF1 taqman probe	Fisher Emergo	Hs00261590_m1
MAFbx taqman probe	Fisher Emergo	Hs01041408_m1
mTOR taqman probe	Fisher Emergo	Hs00234508_m1
p70S6K taqman probe	Fisher Emergo	Hs00177357_m1
cATG12 taqman probe	Fisher Emergo	Hs01047860_g1
LC3b taqman probe	Fisher Emergo	Hs00797944_s1
Lat1SLC taqman probe	Fisher Emergo	Hs00185826_m1
PAT1 taqman probe	Fisher Emergo	Hs01092773_m1
SNAT2 taqman probe	Fisher Emergo	Hs01089954_m1
CD98 taqman probe	Fisher Emergo	Hs00374243_m1
Beclin1 taqman probe	Fisher Emergo	Hs00186838_m1
Myostatin taqman probe	Fisher Emergo	Hs00193363_m1
PGC1-alpha taqman probe	Fisher Emergo	Hs01016719_m1
18S taqman probe	Fisher Emergo	Hs03003631_g1
Critical commercial assays
Glucose HK CP	ABX Pentra	A11A01667
Insulin Elisa kit	Meso Scale Discovery	K151BZC-3
Software
Image Studio 5.2	LI-COR biosciences	5.2
Prism	GraphPad Software	v7.0a
SPSS	IBM	27.0.1.0
BioRender	BioRender	https://www.biorender.com/