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Cellular effect of the thermosensitive mutation. Strain JH17041 was grown in LB broth at 30°C to an OD525 of 0.35, when half the culture was shifted at 47°C. Phase-contrast images of cells taken 1.5 h after the shift are shown. A, 30°C (OD525 = 1.7); B, 47°C (OD525 = 0.6).
