TABLE 1.
Bacterial strains and plasmids used in this study
| B. subtilis straina or plasmid | Genotype or descriptionb | Sourcec |
|---|---|---|
| JH642 | pheA1 trpC2 | Laboratory stock |
| JH646 | pheA1 trpC2 spo0A12 | Laboratory stock |
| JH17022 | amyE::(yycF′-lacZ cat) | pJC16→JH642 |
| JH17023 | yycF::(yycF′-lacZ cat) | pJC17→JH642 |
| JH17024 | spo0A12 amyE::(yycF′-lacZ cat) | JH17022 DNA→JH646 |
| JH17025 | spo0A12 yycF::(yycF′-lacZ cat) | JH17023 DNA→JH646 |
| JH17026 | yycH::pJC11 (Cmr) | pJC11→JH642 |
| JH17027 | yycI::pJC12 (Cmr) | pJC12→JH642 |
| JH17028 | yycJ::pJC13 (Cmr) | pJC13→JH642 |
| JH17029 | yycK::pJC14 (Cmr) | pJC14→JH642 |
| JH17040 | yycF::(yycF′-lacZ Err)-PspacyycFGHIJK | pJC21→JH642 |
| Mu8u5u16 | ade-16 leu-8 met-5 | Laboratory stock |
| JH17041 | leu-8 met-5 yycF (H215P) (temperature-sensitive strain) | 5.4-kb PCR→Mu8u5u16 |
a
All JH strains were derived from JH642.
b
cat is the chloramphenicol acetyltransferase gene from pC194. Plasmid-encoded antibiotic resistance: Cmr, chloramphenicol resistance; Err, erythromycin resistance.
c
Arrows indicate construction by transformation.