Figure 4.

Human MLL-AF9⁺ acute myeloid leukemia cells are sensitive to TWIST1 knockdown. (A, B) Effects of TWIST1 knockdown on cell proliferation (A) and colony formation (B) in MOLM-13 and THP-1 cells, as determined by cell counting and colony-forming assay, respectively (N=3-5). (C-E) MOLM-13-luc2 cells were transfected with TWIST1 small hairpin RNA (shRNA) or control scramble shRNA and engrafted into NOD/SCID mice (8×10⁵ cells per mouse). Bioluminescence imaging of representative mice from each group was taken on day 7 and day 14 post-transplantation (C). Quantification of the bioluminescence imaging at the indicated time points (D, N=3-4). Kaplan-Meier survival curves of MOLM13-transplanted mice (E, N=8). Data are represented as mean ± standard deviation. *P<0.05; **P<0.01; ***P<0.001, by Student’s t test (A, B and D) or log-rank test (E).