Figure 4.

TAK-981+5-azacytidine induces apoptosis and cell cycle defects in acute myeloid leukemia cells. (A) U937 or THP1 cells were treated every day for 3 consecutive days with TAK-981, 5-azacytidine (AZA) or venetoclax (VEN) at the indicated doses and stained on day 4 with Annexin-V/7AAD to quantify apoptotic and dead cells (N=3, mean +/- standard deviation [SD], conditions were compared to mock-treated condition using ordinary one-way ANOVA). (B) Cells treated as in (A) were stained with propidium iodide to analyze cell cycle distribution (N=3, mean +/- SD).