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. 2023 Dec 21;12(12):1871–1884. doi: 10.21037/tau-23-573

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2023 Translational Andrology and Urology. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Effect of Klotho treatment on ferroptosis in CLP-induced SA-AKI. (A) Representative IF images and (B) quantification of GPX4 (red) in mouse kidneys from each group. n=3. (C) IF representative images and (D) quantification of Nrf2 (green) from mice in each group. n=3. (E) Levels of iron in tissue. n=6. The results are expressed as the means ± standard deviation. *, P<0.05; **, P<0.01. CLP, cecal ligation and puncture; Lip-1, liprostatin-1; Klotho, recombinant human Klotho protein; DAPI, 4,6-diamino-2-phenyl indole; Nrf2, nuclear factor erythroid-2 related factor 2; SA-AKI, sepsis-associated acute kidney injury; IF, immunofluorescence; ns, no significance.