FIG. 2.

DNA region of S. liquefaciens PL10 (including size markers) containing the transposon insert. The extent of cloned and sequenced DNA on plasmids pPL1051 and pPL1052 is indicated by arrows. The predicted SwrA ORF of 911 amino acids (aa) is shown as a thick arrow. Homology between the putative SwrA and the SrfA surfactin synthetase subunits Srf1 and Srf2 of B. subtilis (7) is as follows: hatched regions of 450 aa are 28% identical and 42% similar and squared regions of 75 aa are 54% identical and 70% similar. The positions of motifs characteristic of peptide synthetases are indicated by black symbols and given by amino acid number. The motif sequences, as well as the specific amino acids (Glu, Leu, etc.) that the corresponding domains bind are shown in the box. Race denotes a domain which catalyzes the l- to d-form epimerization of the adjacent bound Leu.