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. 2023 Nov 30;300(1):105511. doi: 10.1016/j.jbc.2023.105511

Figure 2.

Figure 2

Effect of missense mutations on C3C6 domain stability.A, expression profiles of WT and HCM variants of C3C6 in bacterial systems. C3C6 is indicated accordingly. Black arrowhead indicates degradation product. B, top, differential scanning fluorimetry unfolding curves for WT and HCM variants of C3C6. Please note that G596R shows increased background fluorescence at low temperature (red arrow). Bottom, first derivative of unfolding curves shown above. G596R and E542Q show an additional transition at lower temperatures (indicated by green and red arrow, respectively). C, summary of measured Tms. Data are presented as means ± SD, n = 5. Statistical significance between WT and HCM variants was assessed with a one-way ANOVA followed by Tukey’s post hoc test. HCM, hypertrophic cardiomyopathy; S, soluble fraction of bacterial lysate; T, total bacterial lysate.