Figure 5.

Co-expression modules and pathway enrichment analyses.A. Co-expression modules. Modules corresponds to sets of genes whose expression is strongly correlated (Pearson r > 0.8). For each module, gene-set variation analysis (GSVA) was performed to generate scores representing expression levels of genes within each module per sample, relative to the other samples. B. Statistical differences in GSVA scores between groups. Colours represent statistical significance from less significant (yellow) to most significant (red). T-test results with p > 0.1 are shown in grey, results with 0.1 < p < 0.05 are shown in yellow with no annotation. Annotations: ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001 (Bonferroni-corrected within modules). C. Over-representation enrichment analyses (KEGG pathways) for genes modules and genes significantly associated with NASH and/or NN1177 (ordinal regression analysis NASH vehicle < NN1177 low < NN1177 med < NN1177 high). The pathways represented are selected from the top 5 pathways per gene-sets. Each gene-set tested is identified by a number in bracket and referenced with this number in the results. The numbers of genes in each gene sets are shown in parenthesis. The variations in number of genes between (A) and (C) are due to differences in gene ID mappings between Ensembl IDs used for expression analyses (A) and Entrez IDs used for enrichment analyses (C). Full differential expression and enrichment results are presented in Suppl. Table 1.