PLS-DA models for calibration by sex of mature Palmer amaranth leaf spectra. “n” refers to the total number of collected spectra per experimental group.

1st run		Actual sex		2nd run	Actual sex
Predicted sex	Accuracy (%)	Female (n = 72)	Male (n = 48)	Accuracy (%)	Female (n = 56)	Male (n = 49)
Female	91.7	66	6	94.6	53	8
Male	87.5	6	42	83.7	3	41
MCC = 0.792				MCC = 0.792