Table 2.

Primers and PCR conditions used in identifying antibiotic resistance genes

Antibiotic group and gene		Primer	Nucleotide sequence(5′–3′)	Product(bp)	Annealing (ºC)	Reference
Sulfonamide	sul1	sul1-F	CGGCGTGGGCTACCTGAACG	433	66	[37]
		sul1-B	GCCGATCGCGTGAAGTTCCG
	sul2	sulII-L	CGGCATCGTCAACATAACCT	721
		sulII-R	TGTGCGGATGAAGTCAGCTC
	sul3	sul3-GKa-F	CAACGGAAGTGGGCGTTGTGGA	244
		sul3-GKa-R	GCTGCACCAATTCGCTGAACG
Quinolone	qnr(A)	QnrAm-F	AGAGGATTTCTCACGCCAGG	580	59	[38]
		QnrAm-R	TGCCAGGCACAGATCTTGAC
	qnr(B)	QnrBm-F	GGMATHGAAATTCGCCACTG	264	59
		QnrBm-R	TTTGCYGYYCGCCAGTCGAA
	qnr(s)	QnrSm-F	GCAAGTTCATTGAACAGGGT	428	55
		QnrSm-R	TCTAAACCGTCGAGTTCGGCG
Tetracycline	tet(A)	TetA-L	GGCGGTCTTCTTCATCATGC	502	63	[37]
		TetA-R	CGGCAGGCAGAGCAAGTAGA
	tet(B)	TetBGK-F2	CGCCCAGTGCTGTTGTTGTC	173	61
		TetBGK-R2	CGCGTTGAGAAGCTGAGGTG
	tet(C)	TetC-L	GCTGTAGGCATAGGCTTGGT	888	58
		TetC-R	GCCGGAAGCGAGAAGAATCA
Aminoglycoside	strA/strB	strA-F	ATGGTGGACCCTAAAACTCT	893	52	[37]
		strB-R	CGTCTAGGATCGAGACAAAG
	aac(3)IV	aac4-L	TGCTGGTCCACAGCTCCTTC	653	55
		aac4-R	CGGATGCAGGAAGATCAA
	aadB	aadB-L	GAGGAGTTGGACTATGGATT	208	52
		aadB-R	CTTCATCGGCATAGTAAAAG
	aphA1	aph(3´)-Ia F	ATGGGCTCGCGATAATGTC	634	58	[39]
		aph(3')-Ia R	CTCACCGAGGCAGTTCCAT
	aphA2	aphA2-L	GATTGAACAAGATGGATTGC	347	53	[37]
		aphA2-R	CCATGATGGATACTTTCTCG

M = A or C; H = A or C or T; Y = C or T