TABLE 1.
Nucleotide content of wild-type and Δprs strains
| Strain | % of nucleotides produceda,b
|
|||
|---|---|---|---|---|
| UXP | CXP | AXP | GXP | |
| YN94-1 (wild type) | 99 ± 0.8 | 100 ± 0.6 | 101 ± 1.5 | 99 ± 1.1 |
| YN96-66 (Δprs1::loxP)c | 25 ± 7.0 | 12 ± 0.5 | 45 ± 9.0 | 37 ± 6.0 |
| YN96-67 (Δprs3::loxP)c | 26 ± 4.0 | 31 ± 9.8 | 55 ± 4.0 | 38 ± 3.0 |
| YN97-7 (Δprs2::loxP)c | 72 ± 13 | 97 ± 1.5 | 91 ± 9.5 | 80 ± 8.2 |
| YN97-6 (Δprs4::loxP)c | 75 ± 15 | 102 ± 2.0 | 70 ± 15 | 86 ± 19 |
| YN96-69 (Δprs5::loxP) | 101 ± 15 | 80 ± 13 | 89 ± 12 | 96 ± 10 |
a
UXP, UMP + UDP + UTP; CXP, CDP + CTP (CMP is not detectable under the experimental conditions used); AXP, AMP + ADP + ATP; GXP, GMP + GDP + GTP.
b
Values are the percentages of nucleotides produced by each strain with respect to that of the wild type and represent the average ± standard deviation of at least three independent determinations.
c
These deletion mutant strains were created as described for Δprs5::loxP in the text by using appropriate primers for each gene.