Skip to main content
. 2023 Dec 18;27(2):59. doi: 10.3892/ol.2023.14192

Figure 2.

Figure 2.

Effect of EGFR on cell proliferation. (A) Western blotting was used to detect the differences in EGFR activation induced by EGF at different times. (B) Western blotting was used to detect the knockdown efficiency of three EGFR knockdown lentiviruses in HepG2 cells. (C) Cell Counting Kit-8 assay and (D) EdU-594 proliferation assay were used to detect the proliferation of HepG2 cells (magnification, ×200). (E) Colony formation assay was used to detect the colony-forming ability of HepG2 cells. *P<0.05, **P<0.01 and ***P<0.001. EGFR, epidermal growth factor receptor; NC, negative control; ns, not significant; p-, phosphorylated; sh, short hairpin.