Fig. 1.

Expression of EVA71 VLPs. (a) EVA71 VLPs were produced in P. pastoris using the dual promoter expression cassette depicted. wt and rs VLPs were purified on 15–45 % sucrose gradients. (b) Fractions were assessed by Western blot using mAb 979, a representative image of n=3 is shown. (c) Fractions were diluted 1 : 10 and heated to 55 °C to facilitate conversion to a HAg conformation. Samples were assessed for reactivity with CT11F9, graph shows mean raw OD 492 nm ±sem, n=3 in duplicate. (d) Unheated fractions were diluted 1 : 10 and assessed for reactivity with HAg-specific mAb 979, graph shows mean raw OD 492 nm ±sem, n=3 in duplicate.