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. 2024 Jan 5;15:324. doi: 10.1038/s41467-023-44382-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Single-cell light chain clonotype analysis using the 10X Genomics platform of FACS-sorted IL-10⁺ (green) and IL-10^– (black) B-1a, marginal zone (MZ), and follicular (FO) B cells from sIgM^–/–IL-10^GFP mouse after 4-h stimulation with phorbol 12-myristate 13-acetate/ionomycin/lipopolysaccharide, gated as in Supplementary Fig. 4. Il10 transcript-expressing cells were removed from GFP^– sorted populations. Data were analyzed with Cell Ranger VDJ Scripts and plotted using R. a The percentage of top five individual clonotypes from each sample is shown with the corresponding CDR3 sequence listed on top. The total cells analyzed and total clonotypes identified are indicated below graphs. Complete information on VJ gene usage and nucleotide sequences found in Supplementary Data 1. b Diversity indexes (Shannon and Inverse Simpson) were assessed for the 6 samples using scRepertoire.