FIG. 1.

Partial restriction map of the 2.8-kb M. xanthus DK101 argE chromosomal region. Arrows indicate putative directions of transcription. The region 3′ to argE including the 3′ end of M. xanthus trcF is shown (for reference, see Garza et al. [4]). Restriction enzymes are abbreviated as follows: A, AccI; B, BamHI; E, EagI; S, SmaI (not all sites shown); S1, SacI. Solid lines indicate DNA regions used for insertion mutagenesis in orf2 (pBAR100) or argE (pBAR113) and expression of argE on the pBluescript lac promoter (pBAR114). A possible ribosome binding site (RBS) and initiator codon (ATG) for argE are shown.