Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1990 Nov;94(3):1111–1115. doi: 10.1104/pp.94.3.1111

Lack of Cross-Resistance of Imidazolinone-Resistant Cell Lines of Datura innoxia P. Mill. to Chlorsulfuron 1

Evidence for Separable Sites of Action on the Target Enzyme

Praveen K Saxena 1,2, John King 1,2
PMCID: PMC1077349  PMID: 16667804

Abstract

Two cell lines of Datura innoxia resistant to two imidazolinone herbicides, imazapyr and imazaquin, were isolated from mutagenized, predominantly haploid cell suspension cultures. Both of the resistant variants were >1000-fold more resistant than the wild-type to the two imidazolinones. The variant resistant to imazapyr showed cross-resistance to imazaquin and vice versa, but no cross-resistance to a structurally different inhibitor, chlorsulfuron, a sulfonylurea herbicide, was observed. The target enzyme, acetolactate synthase, extracted from imidazolinone-resistant cell lines was not inhibited by imazapyr or imazaquin but was sensitive to chlorsulfuron indicating separable sites of action for these inhibitors. The variation in resistance and cross-resistance of chlorsulfuron-resistant (PK Saxena, J King [1988] Plant Physiol 86: 863-867) and imidazolinone-resistant cell lines of Datura innoxia demonstrates the possibility of separate mutations of acetolactate synthase gene resulting in specific phenotypes.

Full text

PDF
1115

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaleff R. S., Mauvais C. J. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science. 1984 Jun 29;224(4656):1443–1445. doi: 10.1126/science.224.4656.1443. [DOI] [PubMed] [Google Scholar]
  2. Chaleff R. S., Ray T. B. Herbicide-resistant mutants from tobacco cell cultures. Science. 1984 Mar 16;223(4641):1148–1151. doi: 10.1126/science.223.4641.1148. [DOI] [PubMed] [Google Scholar]
  3. Falco S. C., Dumas K. S. Genetic analysis of mutants of Saccharomyces cerevisiae resistant to the herbicide sulfometuron methyl. Genetics. 1985 Jan;109(1):21–35. doi: 10.1093/genetics/109.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gabard J. M., Charest P. J., Iyer V. N., Miki B. L. Cross-resistance to short residual sulfonylurea herbicides in transgenic tobacco plants. Plant Physiol. 1989 Oct;91(2):574–580. doi: 10.1104/pp.91.2.574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  6. Lee K. Y., Townsend J., Tepperman J., Black M., Chui C. F., Mazur B., Dunsmuir P., Bedbrook J. The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO J. 1988 May;7(5):1241–1248. doi: 10.1002/j.1460-2075.1988.tb02937.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Muhitch M. J., Shaner D. L., Stidham M. A. Imidazolinones and acetohydroxyacid synthase from higher plants: properties of the enzyme from maize suspension culture cells and evidence for the binding of imazapyr to acetohydroxyacid synthase in vivo. Plant Physiol. 1987 Feb;83(2):451–456. doi: 10.1104/pp.83.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ray T. B. Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol. 1984 Jul;75(3):827–831. doi: 10.1104/pp.75.3.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rost T. L., Reynolds T. Reversal of chlorsulfuron-induced inhibition of mitotic entry by isoleucine and valine. Plant Physiol. 1985 Feb;77(2):481–482. doi: 10.1104/pp.77.2.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saxena P. K., King J. Herbicide Resistance in Datura innoxia: Cross-Resistance of Sulfonylurea-Resistant Cell Lines to Imidazolinones. Plant Physiol. 1988 Mar;86(3):863–867. doi: 10.1104/pp.86.3.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shaner D. L., Anderson P. C., Stidham M. A. Imidazolinones: potent inhibitors of acetohydroxyacid synthase. Plant Physiol. 1984 Oct;76(2):545–546. doi: 10.1104/pp.76.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sigematsu Y., Sato F., Yamada Y. The mechanism of herbicide resistance in tobacco cells with a new mutation in the q(b) protein. Plant Physiol. 1989 Mar;89(3):986–992. doi: 10.1104/pp.89.3.986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES